Electrochemical aptasensor for detecting tetracycline in milk
نویسندگان
چکیده
منابع مشابه
Electrochemical aptasensor for tetracycline detection.
An electrochemical aptasensor was developed for the detection of tetracycline using ssDNA aptamer that selectively binds to tetracycline as recognition element. The aptamer was highly selective for tetracycline which distinguishes minor structural changes on other tetracycline derivatives. The biotinylated ssDNA aptamer was immobilized on a streptavidin-modified screen-printed gold electrode, a...
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Developing the rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper, we developed a label-free aptasensor for electrochemical detection of tetracycline. The reorganization of tetracycline binding aptamer was confirmed by Isothermal Titration Calorimetry, Kd 1⁄4 5.18 10 5 mol L 1. According to the electrochemical impendence spect...
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A bifunctional derivative of the thrombin-binding aptamer with a redox-active Fc moiety and a thiol group at the termini of the aptamer strand was synthesized. The ferrocene-labeled aptamer thiol was self-assembled through S-Au bonding on a polycrystalline gold electrode surface and the surface was blocked with 2-mercaptoethanol to form a mixed monolayer. By use of a fluorescent molecular beaco...
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ژورنال
عنوان ژورنال: Advances in Natural Sciences: Nanoscience and Nanotechnology
سال: 2016
ISSN: 2043-6262
DOI: 10.1088/2043-6262/7/1/015008